building blocks of life
Copyright © 2009 - 2016 ACES Pharma Inc. All rights reserved.
Despite much progress, the development of new and improved cancer
therapies remains one of the greatest medical challenges. In addition to
the continuous refinement of already well-established treatments with
surgery, radiation and chemotherapy, immunological approaches have
been gaining in interest and efficacy and are becoming important
alternatives or adjuncts to conventional therapy for several types of tumors.

Our Technology and Service:

  • Provides a unique fluorescent dye(AC dye) for different antigens
  • Unique fluorescent labeling technology
  • Labeled antigen, antibodies and cytokines of T cells, NK cells and
    other immune cells
  • Provides the analysis of antigen, cytokine from immune cell and
    provide the best marker dye
  • Unique ELISPOT, FluoroSpot kit etc
Our company based on the characteristics (such as density) of antigen
or cytokine from T cells, NK cells and other immune cells, to custom
synthesis the corresponding fluorescent dyes and develop the best color
scheme for you, to help you better understand the experimental results.
Detection of T cells reacted to tumor

As a consequence of antigen recognition,T cells will be stimulated to produce
cytokines and to express activation markers. T cells and their subsets can be
identified by differential expression of cell surface markers including CD3,
CD4, CD8, CD25, CD127, and CD19 (CCR6).This information can be used to
identify different memory subsets.
Cell Surface Markers to identify cells from heterogenous
Intracellular Cytokin Staining to measure cytokines within
individual cells;
AC Phosflow technology to measure the phosphorylation of
key proteins;
AC Cytometic Array to measure secreted cytokines within a

Classification of antigen
  • The first type, easy to distinguish the masculine cegroup,
    such as CD3,CD4,CD45
  • The second type, the high level expression,such as CD27,
    CD28, CD45RA, CD45RO
  • The third type,expressed at low levels, activation marker,
    the expression is not obvious distinction, such as CD25
Antigen with the lowest expressing density matching the
brightest fluorescein

Select of fluorescein and antigen
  • Select the most appropriate fluorescein-conjugated
  • Characteristics of antigen expression
  • With the availability of multiple AC dyes,more color
    schemes can be created.

Importance of dye selection

The suitable fluorescein will help us to understand the
experiment better, for weak antigens, bright dyes are
particularly important. Choosing the right combination of
fluorescence is the key to answer biological questions.

  • Low density antigen needs brighter fluorescein
  • Minimum overlap between the fluorescent dye
  • Avoid the false positive result from coupling dye
Characteristics of AC dye
  • Ex Max:491 nm
  • Em Max:514 nm
  • Alternatives:FITC,AC-F514 is up to eight times brighter
    than FITC

Applications of AC dye (purple dye for example)

T cells laser excitation:
  • Based polymer: AC-F420, AC-F511; Series body:
   AC-F651, AC-F710 and AC-F785
  • Brighter dyes
  • Reduce cross-laser excitation
  • Reduce the impact of sample preparat

  • Low density antigen needs brighter fluorescein
  • Minimum overlap between the fluorescent dye
  • Avoid the false positive result from coupling dye.
Contitued, Please go to next page for NK cells, EliSpot and
FluoroSpot technology